²úÆ·ÃèÊö
¿ìËÙÀ©ÔöcDNAÄ©¶Ë£¨RACE£©ÊÇÒ»ÖÖµ±RNAת¼±¾µÄÐòÁнö²¿·ÖÒÑ֪ʱ£¬ÒÔRNAΪģ°å¸ßЧÄæתȫ³¤cDNA£¬²¢½«cDNA×÷Ϊģ°å¿ìËÙÀ©ÔöÆä5’Ä©¶Ë¡£ÊÔ¼ÁºÐ°üº¬5’RACEÀ©ÔöËùÐè×é·Ö£¬¿É¸ù¾ÝʵÑéÐèÇó½øÐÐÑ¡Ôñ¡£
²úÆ·×é³ÉÓë´¢´æÌõ¼þ£¨10 rxns£©
| ÊÔ¼ÁÃû³Æ |
Ìå»ý£¨¦ÌL£© |
´¢´æÌõ¼þ |
| dNTPs |
20 |
-20¡æ |
| 5′ Primer A |
10 |
-20¡æ |
| Murine RNase Inhibitor |
4 |
-20¡æ |
| DTT |
10 |
-20¡æ |
| Reverse Transcriptase |
4 |
-20¡æ |
| 2 ¡Á PCR Mix |
250 |
-20¡æ |
| RNase- Free water |
1000 |
-20¡æ |
| 10 ¡Á UPM |
50 |
-20¡æ |
| TS Primer |
10 |
-80¡æ |
| 5 ¡Á RT Buffer |
48 |
-80¡æ |
×Ô±¸²ÄÁÏ
- ×ÜRNA»òÕßmRNAÑù±¾£»
- Õñµ´Æ÷¡¢Ë²Ê±ÀëÐÄ»ú¡¢PCRÒǵȣ»
- 5′ GSP Primer¡£
×¢ÒâÊÂÏî
- PCR²Ù×÷¾ùÔÚ±ùÉϽøÐУ»
- ËùÓÐPCRÊÔ¼Á¼°ºÄ²ÄÐèÎÞøÎÞ¾ú¡£
ÎïÁ÷ƽ̨£ºÄ¬ÈÏΪ¾©¶«ÎïÁ÷¡£ÈôÓÐÆäËûÐèÇó£¬ÇëÁªÏµ¶Ô½ÓÏúÊÛ¡£
·¢»õʱ¼ä£ºÈ·È϶©µ¥²¢Íê³É¸¶¿îºó1~2¸ö¹¤×÷ÈÕÄÚ°²ÅÅ·¢»õ¡£
·¢»õ·½Ê½£º±ù´ü»ò¸É±ù¡£
ËÕ¹«Íø°²±¸32011202001300
ËÕICP±¸2021019379ºÅ-1
|